stability of Brevibacterium CGTase, but it did not affect the heat stability of the other CGTases tested. Bacillus subtilis CGTase produced y-CD and mal-.

1999-12-03

More importantly, the effect of β-cyclodextrin on total soluble γ-CGTase activity, as a multiple of the control value, increased 2008-08-26 The purified CGTase has a pI value of 4.2. The optimum pH of 6.0 and 60 °C temperature were found to be the best for CGTase activity. Purified CGTase showed 5.18 kcal/mol activation energy (Ea). Objectives: Cyclodextrin glucanotransferase (CGTase) is a multifunctional industrial enzyme, which undergoes cyclization reaction to converts starch into Cyclodextrin (CD).

Paenibacillus pabuli. Status. Unreviewed-Annotation score: -Protein inferred from homology i. Function i Cofactor i.

The enzymes from the α-amylase family all share a similar α-retaining catalytic mechanism but can have different reaction and product specificities. One family member, cyclodextrin glycosyltransferase (CGTase), has an uncommonly high transglycosylation activity and is able to form cyclodextrins. We have determined the 2.0 and 2.5 Å X-ray structures of E257A/D229A CGTase in complex with

The purified Y93F CGTase was 76.39 kDa based on 10% SDS-PAGE analysis and showed both β-cyclization and starch hydrolysis activities in a zymography assay. There was no major structural The CGTase enzyme obtained from the M5 clone had Km and Vmax values 13.59 mg/mL and 1153 µmol/(mg min), respectively.

Cyclodextrin glycosyl transferase (CGTase, EC 2.4.1.19) is an important industrial enzyme, unique in its ability to convert starch and related glycans into non-reducing, cyclic malto-oligosacchrarides called cyclodextrins (CDs) via a cyclization reaction, an intramolecular transglycosylation reaction [1].

… CGTase, was selected from B. cereus YUPP-10 by a constructed fos-mid library. We discovered that CGTase has antimicrobial activity and induces resistance.

Cyclodextrin glucanotransferases (CGTase; E.C 2.4.1.19) belonging to the glycoside hydrolase family 13 (GH 13) are widely used as catalysts in starch conversion processes (Han et al.
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The enzyme cyclomaltodextrin-glucanotransferase (CGTase) is a transglicosidase able to convert corn starch into cyclodextrin (CD).

In 2001, a novel food application was submitted to the Italian novel food Competent Substrate complexation and aggregation influence the cyclodextrin glycosyltransferase (CGTase) catalyzed synthesis of alkyl glycosides. Zehentgruber, Daniela LU; Lundemo, Pontus LU; Svensson, David LU and Adlercreutz, Patrick LU () In Journal of Biotechnology 155. p.232-235 Fig. 1 Effect of chemical chaperone candidates on γ-CGTase expression in E. coli.
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This book deals with Production of cyclodextrin glucanotransferase (CGTase) from Bacillus subtilis isolated from agriculture field soil samples. Initial screening

One family member, cyclodextrin glycosyltransferase (CGTase), has an uncommonly high transglycosylation activity and is able to form cyclodextrins.

cgtase cyclodextrin amino acid bacillus amino Prior art date 1990-03-27 Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Expired - Lifetime Application number US07/927,316 Inventor Gerhard Schmid

B. circulans, B. macerans and B. stearothermophilus) and Brevibacillus brevis. [1] This enzyme belongs to the family of glycosyltransferases , specifically the hexosyltransferases.

Organism. Anaerobranca gottschalkii. Status. Unreviewed-Annotation score: -Protein inferred from homology i. Function i Cofactor i. Ca 2+ ARBA annotation. GO Commonly cyclodextrin glycosyltransferase (CGTase) is employed along with α-amylase.